Summary
Wolbachia infecting the small brown planthopper, Laodelphax striatellus, were successfully maintained and cultivated in two insect and one mammalial cell lines. The bacteria with the planthopper ovary were introduced into the flasks with the cultures of the cell lines. The Wolbachia proliferated in mosquito (Aedes albopictus) and lepidopteran (Heliothis zea) cell lines and in the mouse cell line, L929. Proliferation of Wolbachia was confirmed by electron microscopy and quantitative polymerase chain reaction. This simple method for the cultivation of Wolbachia was applicable to other strains of Wolbachia, such as the one found in the lepidopteran eggs, and should facilitate fundamental and applied studies of this important group of microorganisms.
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Noda, H., Miyoshi, T. & Koizumi, Y. In vitro cultivation of Wolbachia in insect and mammalian cell lines. In Vitro Cell.Dev.Biol.-Animal 38, 423–427 (2002). https://doi.org/10.1290/1071-2690(2002)038<0423:IVCOWI>2.0.CO;2
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DOI: https://doi.org/10.1290/1071-2690(2002)038<0423:IVCOWI>2.0.CO;2